Coding
Part:BBa_K898890
Designed by: Hsiao-Han Chen. Anthony Chau. Uros Kuzmanovic. Zefeng Wang. Group: iGEM12_UIUC_Illinois (2012-09-30)
wtPUF+PIN
This part encodes two protein domains: a PUF domain that recognized 8 RNA bases: UGUAUAUA and a PIN domain that cleaves two sites near the 8 bases (the third base within the 8 bases counted from 5'-3' ; the fourth base downstream the 8 bases counted from 5'-3')
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 1297
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 35
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
excitation |