Coding

Part:BBa_K898890

Designed by: Hsiao-Han Chen. Anthony Chau. Uros Kuzmanovic. Zefeng Wang.   Group: iGEM12_UIUC_Illinois   (2012-09-30)


wtPUF+PIN

This part encodes two protein domains: a PUF domain that recognized 8 RNA bases: UGUAUAUA and a PIN domain that cleaves two sites near the 8 bases (the third base within the 8 bases counted from 5'-3' ; the fourth base downstream the 8 bases counted from 5'-3')

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1297
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 35
  • 1000
    COMPATIBLE WITH RFC[1000]


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